Dehydroascorbic Acid Colorimetric Assay Kit

Dehydroascorbic Acid Colorimetric Assay Kit

• Field of Research: Plant Physiology Series
• Detection Method: Colorimetric method
• Assay Principle: Ascorbic acid (AsA) is an important physiological index of plant cells. The changes of its content, redox status (AsA/DHA ratio), and the activities of enzymes related to its synthesis and metabolism are involved in the response of plants to a series of environmental stresses. Dehydroascorbic acid (DHA) is a reversible oxidized form of AsA. In vivo, dehydroascorbic acid (DHA) and ascorbic acid form a redox system and act as an electron acceptor.
  The detection principle of this kit is that DTT reduces DHA to produce AsA, and the DHA content can be calculated by measuring the formation rate of AsA in the system at 265 nm.
• Assay Performance Time: 40 min
• Sample Type: Plant tissue
• Detection Range: 0.03-1.00 μmol/mL
• Shipping Conditions: Ice bag
• Storage Conditions: This product can be stored at 2-8°C for 12 months with shading light.
• Applications Notes: Quantitative