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Glutamic Acid Colorimetric Assay Kit

CAT: 0763-E-BC-K118-S-01Size: 50 Assays (46 samples)Dry Ice: NoHazardous: No
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CAT#:0763-E-BC-K118-S-01Size:50 Assays (46 samples)
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Type
Colorimetric method
Field of Research
Amino Acids and Proteins; Ferroptosis
Assay Principle
Glutamic acid can react with NAD+ under the catalysis of glutamate dehydrogenase to produce α-ketoglutaric acid, NADH and NH4+. NADH has the maximum absorption at 340 nm. And glutamic acid content can be calculated by measuring the change of NADH.
Assay Performance Time
60 min
Sample Type
Serum; plasma; animal tissue; cells; cell culture supernatant
Detection Range
4.00-450 μmol/L
Sensitivity
4.00 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Citation 01
https://www.sciencedirect.com/science/article/pii/S0014299922005659
Inter CV
2.9%
Intra CV
2.2%
Detection Instrument
Spectrophotometer
Product Datasheet
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