Recombinant Mouse FGF2

• Product Name Alternative:

  Basic fibroblast growth factor, Basic fibroblast growth factor bFGF, BFGF, FGF 2, FGF B, FGF-2, Fgf2, FGF2 basic, FGF2_HUMAN, FGFB, Fibroblast growth factor 2

• UniProt:

  P15655

• Expression Region:

  10-154

• Host:

  E.Coli

• Tag:

  N-terminal His Tag

• Applications:

  Western Blot, ELISA

• Endotoxin:

  < 1 EU/µg as determined by LAL test.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 0.6-2 ng/mL.

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  15.8 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

• Organism Species:

  Mouse