Anti-Hu CD32 APC

Anti-Hu CD32 APC

• Background: CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).
• Specifications: The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: purified glycosylated recombinant human FcgammaRIIa2
• Target Antigen: CD32
• Isotype: Igg1 κ
• Clone: 3D3
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Dutertre CA, Bonnin-Gélizé E, Pulford K, Bourel D, Fridman WH, Teillaud JL: A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function. J Leukoc Biol. 2008 Dec84(6):1511-20, URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=pulford+k+bourel+d ,*Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tj248nnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 154(3):566-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/23933259,*Dai X, Jayapal M, Tay HK, Reghunathan R, Lin G, Too CT, Lim YT, Chan SH, Kemeny DM, Floto RA, Smith KG, Melendez AJ, MacAry PA: Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa. Blood. 2009 Jul 9114(2):318-27., URL: https://pubmed.ncbi.nlm.nih.gov/19420354/,*Vely F, Gruel N, Moncuit J, Cochet O, Rouard H, Dare S, Galon J, Sautes C, Fridman WH, Teillaud JL: A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays. Hybridoma. 1997 Dec16(6):519-28., URL: https://pubmed.ncbi.nlm.nih.gov/9455704/,*Boruchov AM, Heller G, Veri MC, Bonvini E, Ravetch JV, Young JW: Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions. J Clin Invest. 2005 Oct115(10):2914-23., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=boruchov+am+heller+g ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.