Rabbit anti Chicken ovalbumin, conjugated with FITC

Rabbit anti Chicken ovalbumin, conjugated with FITC

• Background: A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilutions of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum. In immunocytochemical and immunohistochemical staining of ovalbumin at the cellular and subcellular level of appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
• CAS Number: 9007-83-4
• Host: Rabbit
• Clone: Polyclonal
• Conjugation: FITC
• Source: Highly purified egg albumin (MW 45,000) isolated from chicken egg whites. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Assay Principle: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
• Form: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7,2); No preservative added; It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Storage Conditions: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the immunoconjugate