Notch1 Antibody (HRP)

CAT: 0013-GTR18028975Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18028975Size:100 µg

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Description

Mouse monoclonal to Notch1 (HRP) . Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N (TM) and a N-terminal fragment N (EC) . Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) . This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane..

Product Name Alternative

Neurogenic locus notch homolog protein 1, hN1, Translocation associated notch protein, TAN1, TAN-1, Motch A, mT14, p300, Motch, Mis6, Neurogenic locus notch protein homolog, NICD, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH Drosophila, homolog of 1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, xotch

UniProt

Q01705

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1

Target

Notch1

Clonality

Monoclonal

Clone

N253/32 (Formerly sold as S253-32)

Conjugation

HRP

Field of Research

Cardiovascular Research

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000)

Molecular Weight

270kDa

Storage Conditions

Conjugated antibodies should be stored according to the product label

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

ICC, IF, WB

NCBI Accession Number

NP_032740.3

Host or Source

Mouse

Preservative

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

Isotype

IgG1

Entrez

18128

UniProtKB · Q01705

Neurogenic locus notch homolog protein 1

NOTC1_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: Q01705
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Notch1
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: Notch 1
EC number: —
Processing: Precursor
Secondary accessions: Q06007, Q3TZW2, Q3U3Y2, Q61905, Q7TQ50, Q7TQ51, Q7TQ52, Q8K428, Q99JC2, Q9QW58, Q9R0X7

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

ActivatorDevelopmental proteinReceptor

Coding sequence diversity

Alternative splicing

Biological process

AngiogenesisDifferentiationNotch signaling pathwayTranscriptionTranscription regulation

Domain

ANK repeatEGF-like domainRepeatSignalTransmembraneTransmembrane helix

Ligand

CalciumMetal-binding

Cellular component

Cell membraneEndosomeMembraneNucleus

PTM

Disulfide bondGlycoproteinHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

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