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VGLUT2 Antibody (HRP)

CAT: 0013-GTR18028816Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18028816Size:100 µg
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Description
Mouse monoclonal to VGlut2 (HRP) . The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic alpha and beta cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI..
Product Name Alternative
VGLUT2, VGluT2, VGLU2, SLC17A6, Solute Carrier Family 17 Member 6, Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter) Member 6, Solute Carrier Family 17 (Vesicular Glutamate Transporter) Member 6, Vesicular Glutamate Transporter 2, DNPI, Differentiation-Associated Na (+) -Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated Na-Dependent Inorganic Phosphate Cotransporter, Differentiation-Associated BNPI, Sodium-Dependent Inorganic Phosphate Cotransporter
UniProt
Q9JI12
Reactivity
Human, Mouse, Rat
Immunogen
Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
Target
VGLUT2
Clonality
Monoclonal
Clone
N29/29 (Formerly sold as S29-29)
Conjugation
HRP
Field of Research
Neuroscience
Purification
Protein G Purified
Concentration
1 mg/ml
Dilution
WB (1:1000), ICC/IF (1:100)
Molecular Weight
60kDa
Storage Conditions
Conjugated antibodies should be stored according to the product label
Notes
For research use only.
Applications Notes
1 μg/ml was sufficient for detection of VGLut2 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Tested Applications
ICC, IF, IHC, WB
NCBI Accession Number
NP_445879.1
Host or Source
Mouse
Preservative
73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
Isotype
IgG1
Entrez
84487

UniProtKB · Q9JI12

Vesicular glutamate transporter 2

VGLU2_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q9JI12
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Slc17a6
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
VGluT2
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Biological process

AntiportIon transportNeurotransmitter transportPhosphate transportSodium transportSymportTransport

Cellular component

Cell membraneCytoplasmic vesicleMembraneSynapseSynaptosome

Ligand

ChlorideSodium

Molecular function

Chloride channelIon channel

PTM

Glycoprotein

Domain

TransmembraneTransmembrane helix

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