AHA1 Protein

• Background:

  Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

• Description:

  Human Recombinant AHA1 Protein

• Product Name Alternative:

  Aha1 Protein, SHSA1 Protein, HSPC322 Protein, p38 Protein

• UNSPSC:

  12352202

• Gene ID:

  10598

• Swiss Prot:

  O95433

• Accession Number:

  NP_036243.1

• Cellular Locus:

  Cytoplasm

• Host:

  E. coli

• Origin Species:

  Human

• Target:

  AHA1

• Conjugation:

  His tag

• Sequence:

  SHMAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTDKLKTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLIFFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEEGVKLLREAMGIYISTLKTEFTQGMILPTMNGESVDPVGQPALKTEERKAKPAPSKTQARPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPATLEADRGGKFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF

• Applications:

  WB, SDS-PAGE, Functional Assay

• Purification Method:

  Affinity Purified

• Concentration:

  Lot/batch specific. See included datasheet.

• Purity:

  >90%

• Weight:

  0.05

• Buffer:

  20mM HEPES buffer pH7.2, 80mM NaCl, 10% glycerol

• Molecular Weight:

  ~38 kDa

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.

• References & Citations:

  1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.