ATG7 Antibody

• Background :

  ATG7 is a pivotal E1-like enzyme essential for autophagosome biogenesis, a core process in cellular homeostasis and neuroprotection. Functioning in concert with ATG10, ATG7 catalyzes the conjugation of ATG12 to ATG5, a critical step in the formation of the autophagosome membrane. Additionally, ATG7 activates ATG8 (LC3/GABARAP family proteins), facilitating their lipidation and incorporation into autophagosomal membranes. This dual role positions ATG7 as a master regulator of autophagic flux. In the context of neuroscience, ATG7 has emerged as a key player in the maintenance of neuronal integrity. Its activity is vital for the clearance of misfolded proteins and damaged organelles—hallmarks of neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's disease. Conditional knockout models have demonstrated that loss of ATG7 in neurons leads to progressive neurodegeneration, underscoring its neuroprotective function. Moreover, ATG7 is critical for metabolic adaptation, particularly in neonates, where it ensures amino acid supply through autophagy during nutrient scarcity. This metabolic role further highlights its importance in brain development and function. Given its central role in autophagy and neuronal survival, ATG7 is a promising therapeutic target in neurodegenerative disease research. Ongoing studies aim to modulate ATG7 activity to restore autophagic balance and mitigate neurodegeneration.

• Description :

  Rabbit Anti-Human ATG7 Polyclonal

• Specifications :

  Detects 77.9 kDa.

• Product Name Alternative :

  ATG7, ATG7_HUMAN, APG7, APG7L, APG7-like, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), ATG7 autophagy related 7 homolog, ATG7 autophagy related 7 homolog (S. cerevisiae), ATG12-activating enzyme E1 ATG7, Ubiquitin-like modifier-activating enzyme ATG7, Ubiquitin-activating enzyme E1-like protein, Ubiquitin activating enzyme E1 like protein, Autophagy-related protein 7, Autophagy related protein 7, Autophagy-related 7 (yeast), Autophagy 7 S. cerevisiae homolog of, GSA7, GSA 7, hAGP7, Atg7l, DKFZp434N0735, 1810013K23Rik, ATG 7, Apg 7

• UNSPSC :

  12352203

• UN Code :

  Non-hazardous

• Hazard Statement :

  Non-hazardous

• Gene ID :

  10533

• Swiss Prot :

  O95352

• Accession Number :

  NP_001129503.2

• Cellular Locus :

  Cytoplasm | Preautophagosomal Structure

• Host :

  Rabbit

• Species Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthetic peptide from the N-terminal of Human ATG7

• Target :

  ATG7

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Tissue Specificity :

  Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

• Type :

  Polyclonal

• Applications :

  WB | ICC/IF | IHC

• Validated Applications :

  WB, ICC/IF, IHC

• Field of Research :

  Cancer | Apoptosis | Autophagy | Cardiovascular System | Heart

• Purification :

  Peptide Affinity Purified

• Detection Range :

  A 1:1000 dilution of SPC-610 was sufficient for detection of ATG7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

• Concentration :

  1 mg/ml

• Dilution :

  WB (1:1000) ; ICC/IF (1:100) ; IHC (1:50) ; optimal dilutions for assays should be determined by the user.

• Weight :

  0.1

• Buffer :

  PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions :

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations :

  1. Mizushima N., et al. (1998) J Biol Chem. 273: 33889-92. 2. Mizushima N., et al. (1998) Nature. 395: 395-8. 3. Suzuki K., et al. (2001) EMBO J. 20: 5971-81. 4. Tanida I., et al. (1999) Mol Biol Cell. 10: 1367-79. 5. Shintani T., et al. (1999) EMBO J. 18: 5234-41.

• Shipping Conditions :

  Blue Ice or 4ºC

• Storage Conditions :

  -20ºC

• Specificity :

  Detects 77.9 kDa.

• Species :

  Human | Mouse | Rat

• CAS Number :

  9007-83-4

• Location :

  Cytoplasm | Preautophagosomal Structure

• Immunogen Species :

  Human