Anti-Lactoferrin Alexa Fluor® 647

Anti-Lactoferrin Alexa Fluor® 647

• Background: Lactoferrin is an iron-binding glycoprotein of the transferrin family, which is released to most biological fluids, with particularly high levels in milk. It has anti-inflammatory (e.g. sequestering of lipopolysaccharides), anti-microbial (e.g. blocking of viral attachment to the target cell), and immunomodulatory properties and can prevent infections in young children. Lactoferrin is considered to bridge the innate and adaptive immune responses. It also participates in iron homeostasis, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Besides biological fluids it is also found in the secondary granules of neutrophils.
• Specifications: The mouse monoclonal antibody LF5-1D2 recognizes lactoferrin, an iron-binding secreted glycoprotein of about 90 kDa, which has anti-inflammatory, immunomodulatory and anti-microbial properties.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: human lactoferrin
• Target Antigen: Lactoferrin
• Isotype: IgG1
• Clone: LF5-1D2
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests. Intracellular staining.
• Validated Applications: FC
• Format: Alexa Fluor® 647
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Zwirzitz A, Reiter M, Skrabana R, Ohradanova-Repic A, Majdic O, Gutekova M, Cehlar O, Petrovčíková E, Kutejova E, Stanek G, Stockinger H, Leksa V: Lactoferrin is a natural inhibitor of plasminogen activation. J Biol Chem 2018 293(22) 8600-8613, URL: https://pubmed.ncbi.nlm.nih.gov/29669808/,
• Other References: *Turin CG, Zea-Vera A, Pezo A, Cruz K, Zegarra J, Bellomo S, Cam L, Llanos R, Casta241eda A, Tucto L, Ochoa TJ NEOLACTO Research Group: Lactoferrin for prevention of neonatal sepsis. Biometals. 2014 Oct27(5):1007-16. doi: 10.1007/s10534-014-9754-3., URL: https://pubmed.ncbi.nlm.nih.gov/24935001/,*Siqueiros-Cendón T, Arévalo-Gallegos S, Iglesias-Figueroa BF, García-Montoya IA, Salazar-Martínez J, Rascón-Cruz Q: Immunomodulatory effects of lactoferrin. Acta Pharmacol Sin. 2014 May35(5):557-66., URL: https://pubmed.ncbi.nlm.nih.gov/24786230/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.