Mouse BMG/b2-MG (Beta-2-Microglobulin) ELISA Kit

Mouse BMG/b2-MG (Beta-2-Microglobulin) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
• UniProt: P01887
• Field of Research: Infection immunity; Kidney biomarker.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 20 µg/mL
• Sample Type: Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.32-20 µg/mL
• Sensitivity: 0.11 µg/mL