AAV-DJ/8 Helper Free Expression System

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1) . AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2. AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

Complete Expression System contains packaging plasmids, expression vector and GFP control vector/pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus/AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9/Cloning capacity of expression vector = 3 kb

41105908

Complete Expression System contains packaging plasmids, expression vector and GFP control vector; pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus; AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9

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Viral Expression->Adeno-Associated Virus (AAV) Expression->AAV Helper Free Complete Expression Systems->AAV-DJ/8 Helper Free Complete Expression Systems