beta 4 Spectrin Antibody

beta 4 Spectrin Antibody

• Background: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2).
• Description: Mouse Anti-Human beta 4 Spectrin Monoclonal IgG1
• Specifications: Detects ~>200kDa. Does not cross react with other Beta-spectrins
• Product Name Alternative: BetaIV-spectrin sigma1 Antibody, Non-erythrocytic spectrin beta chain 4 Antibody, Non-erythroidspectrin beta chain 3 Antibody, SPTBN4 Antibody, SPTBN3 Antibody, Spnb4 Antibody, KIAA1642 Antibody, EMBL BAB83243.1 Antibody, Ensembl ENSRNOP00000028393 Antibody
• Synonyms: beta 4 Spectrin Antibody, Clone N393/2
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Gene ID: 57731
• Swiss Prot: Q9H254
• Accession Number: NP_066022.2
• Cellular Locus: Cytoplasm | Cytoskeleton | Cytoplasm | Cell Cortex
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin
• Target: beta 4 Spectrin
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: N393/2 (Formerly sold as S393-2)
• Conjugation: Unconjugated
• Validated Applications: WB, IHC, ICC/IF
• Purification: Protein G Purified
• Limit Of Detection: A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer changes when conjugated
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Genes and mapped phenotypes. (n.d.). Retrieved March 12, 2015, from http://www.ncbi.nlm.nih.gov/gene/57731 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24(33): 7230-7240.