Beta-4 Spectrin Antibody, Clone N393/2: ATTO 594

• Background:

  Beta IV spectrin (SPTBN4) is a non-erythrocytic member of the spectrin family, a group of cytoskeletal proteins essential for maintaining cell shape, membrane integrity, and the spatial organization of intracellular components. While spectrins are best known for their role in red blood cells, beta IV spectrin is predominantly expressed in the nervous system, particularly in the cerebellum, spinal cord, and brain, as well as in pancreatic islets. In neurons, beta IV spectrin localizes to the nuclear matrix, cytoplasmic vesicles, and promyelocytic leukemia (PML) nuclear bodies. It plays a critical role in stabilizing membrane domains and organizing ion channels at the nodes of Ranvier—specialized axonal segments essential for saltatory conduction. This structural role is vital for maintaining the fidelity of neuronal signaling. Disruption of beta IV spectrin function has been linked to neurodevelopmental and neurodegenerative disorders. Mutations in the SPTBN4 gene are associated with severe neurological phenotypes, including motor dysfunction, intellectual disability, and epilepsy. Given its role in axonal architecture and signal propagation, beta IV spectrin is emerging as a key player in the pathophysiology of diseases such as multiple sclerosis, peripheral neuropathies, and potentially ALS. As a cytoskeletal scaffold critical for neural connectivity and excitability, beta IV spectrin represents a promising target for research into the mechanisms underlying neurodegeneration and for the development of neuroprotective strategies.

• Description:

  Mouse Anti-Human Beta-4 Spectrin Monoclonal IgG1

• Specifications:

  Detects ~>200kDa. Does not cross react with other Beta-spectrins

• Product Name Alternative:

  Beta-IV spectrin, Spectrin beta chain, non-erythrocytic 4, Spectrin, non-erythroid beta chain 3, SPTBN4, SPTBN3, Spnb4, KIAA1642, EMBL BAB83243.1, Ensembl ENSRNOP00000028393

• Synonyms:

  beta 4 Spectrin Antibody, Clone N393/2: ATTO 594

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  57731

• Swiss Prot:

  Q9H254

• Accession Number:

  NP_066022.2

• Cellular Locus:

  Cytoplasm | Cytoskeleton | Cytoplasm | Cell Cortex

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin

• Target:

  beta 4 Spectrin

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  N393/2 (Formerly sold as S393-2)

• Conjugation:

  ATTO 594

• Type:

  Monoclonal

• Applications:

  WB | IHC | ICC/IF

• Validated Applications:

  IHC, ICC/IF

• Field of Research:

  Neuroscience | Cell Signaling | Scaffold Proteins

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Genes and mapped phenotypes. (n.d.). Retrieved March 12, 2015, from http://www.ncbi.nlm.nih.gov/gene/57731 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24(33): 7230-7240.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects ~>200kDa. Does not cross react with other Beta-spectrins

• Background Reference 01:

  1. Genes and mapped phenotypes. (n.d.) . Retrieved March 12, 2015, from http://www.ncbi.nlm.nih.gov/gene/57731 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24 (33) : 7230-7240.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm | Cytoskeleton | Cytoplasm | Cell Cortex

• Isotype:

  IgG1

• Immunogen Species:

  Human