Uncoated Mouse PDGF-BB ( Platelet Derived Growth Factor BB ) ELISA Kit

Uncoated Mouse PDGF-BB ( Platelet Derived Growth Factor BB ) ELISA Kit

• Abbreviation: PDGF-BB
• Synonyms: c-sis; IBGC5; PDGF2; PDGF-2; PDGFB; Platelet-derived growth factor subunit B; SIS; SSV; PDGFBB; PDGF-BB
• CAS Number: 7732-18-5
• UniProt: P31240
• Cross Reactivity: This kit recognizes PDGF-BB in samples.No significant cross-reactivity or interference between PDGF-BB and analogues was observed.
• Target: Mouse
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cell Biology; Immunology; Neuroscience; Stem Cells
• Detection Method: Colorimetric method; ELISA; Sandwich
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDGF-BB , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PDGF-BB . You can calculate the concentration of PDGF-BB in the samples by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 24 h
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 15.63-1000 pg/mL
• Precision: Both intra-CV and inter-CV are < 10%
• Recovery: 80%-120%
• Shipping Conditions: Ice bag
• Storage Conditions: -20°C,12 months
• Applications Notes: This ELISA kit applies to the in vitro quantitative determination of Mouse PDGF-BB concentrations in serum, plasma and other biological fluids.