HSP70 Antibody

HSP70 Antibody

• Background: HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
• Description: Mouse Anti-Human HSP70 Monoclonal IgG1
• Specifications: Detects ~70kDa. May detect HSP70, HSC70, p75 and HSP72.
• Product Name Alternative: HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
• Synonyms: HSP70 Antibody, Clone 3A3
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Gene ID: 3303
• Swiss Prot: P0DMV8/P0DMV9
• Accession Number: NP_005336.3
• Cellular Locus: Cytoplasm
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat, Amphibians, Chicken, Fish, Yeast, Drosophila, Bacteria, Brine Shrimp
• Immunogen: Human recombinant HSP70 overexpressed in E.coli
• Target: HSP70
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 3A3
• Conjugation: Unconjugated
• Validated Applications: WB, IHC, ICC/IF, IP, AM
• Purification: Protein G Purified
• Limit Of Detection: 0.2 µg/ml of SMC-164 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:5000), ICC/IF (1:500), IP (2µg); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Balashova N. et al. (2005) J Biol Chem 280:2186-96. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nat Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.