P38 alpha MAPK Antibody, Clone 9F12: ATTO 488

• Background:

  P38 MAPK (also known as SAPK2α) is a stress-activated kinase that orchestrates cellular responses to inflammatory and oxidative stimuli. It is activated by phosphorylation in response to diverse stressors, including hydrogen peroxide, cytokines, UV light, and chemotherapeutic agents. In neurons, p38 MAPK regulates apoptosis, synaptic remodeling, and inflammatory gene expression. Its activation leads to mitochondrial cytochrome c release and caspase-3 activation, contributing to neurodegeneration. Elevated p38 MAPK activity has been observed in Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis. Given its central role in stress signaling and cell death, p38 MAPK is a validated target for therapeutic intervention in neurodegenerative and inflammatory CNS disorders.

• Description:

  Mouse Anti-Human p38 alpha MAPK Monoclonal IgG1

• Specifications:

  Detects ~38kDa.

• Product Name Alternative:

  CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, MAPK14, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A

• Synonyms:

  p38 alpha (MAP Kinase) Antibody, Clone 9F12: ATTO 488

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  1432

• Swiss Prot:

  Q16539

• Accession Number:

  NP_001306.1

• Cellular Locus:

  Cytoplasm | Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Full length recombinant protein expressed in E.coli cells

• Target:

  p38 MAPK

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  9F12

• Conjugation:

  ATTO 488

• Type:

  Monoclonal

• Applications:

  WB | IHC | IP | ELISA | AM

• Validated Applications:

  IHC, ICC/IF

• Field of Research:

  Cell Signaling | Post-translational Modifications | Phosphorylation | Cancer

• Purification:

  Protein G Purified

• Limit Of Detection:

  Detects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:1000) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.318(1): 99-107.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  Conjugated antibodies should be stored according to the product label

• Specificity:

  Detects ~38kDa.

• Background Reference 01:

  1. Pearson, G. et al (2001) . Endocrine Reviews 22 (2) : 153-183. 2. Fan, Y. et al (2007) Mol. Cells 23 (1) : 30-38. 3. Han, J. et al. (1994) Science 265: 808-811. 4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell. 115: 61-70. 6. Salojin KV, et al. (2006) J Immunol. 176 (3) :1899-907. 7. Medicherla S. et al. (2006) . J Pharmacol Exp Ther.318 (1) : 99-107.

• Species:

  Human | Mouse | Rat

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm | Nucleus

• Isotype:

  IgG1

• Immunogen Species:

  Human