HSP27 Antibody, Clone 8A7

CAT: 0400-SMC-114SSize: 12 µgDry Ice: NoHazardous: No
CAT#:0400-SMC-114SSize:12 µg
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Background
HSP25 (mouse) and its human homolog HSP27 are members of the small heat shock protein (sHSP) family, characterized by a conserved α-crystallin domain and a variable N-terminal region essential for oligomerization. These proteins form dynamic oligomers ranging from dimers to large multimers (8–40 monomers), with chaperone activity closely tied to their oligomeric state—larger assemblies exhibit potent anti-aggregation functions, while dimers are inactive. HSP27 is predominantly cytoplasmic under basal conditions but rapidly translocates to the nucleus in response to cellular stress, where it may stabilize nuclear structures and DNA. It is also rapidly phosphorylated in response to various stimuli, linking it to second messenger signaling pathways. Functionally, HSP27 acts as an ATP-independent molecular chaperone, preventing protein aggregation and stabilizing partially unfolded proteins, often in coordination with the HSP70 complex. In the nervous system, HSP27 plays a critical role in protecting neurons from proteotoxic stress, apoptosis, and oxidative damage—key features of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. It inhibits apoptotic signaling by blocking the cytochrome c/Apaf-1/procaspase-9 complex and may also influence cytoskeletal dynamics through interactions with actin and myosin. Upregulation of HSP27 correlates with increased phosphorylation and oligomer formation, suggesting a role in stress adaptation, cell differentiation, and potentially growth arrest. These properties make HSP27 a compelling target for therapeutic strategies aimed at enhancing neuronal resilience in neurodegenerative disease.
Description
Mouse Anti-Human HSP25/HSP27 Monoclonal IgG1 Kappa
CAS Number
9007-83-4
Specifications
Detects ~25kDa or ~27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Product Name Alternative
HSPB1, HSP27, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, MKBP, DMPK-binding protein
Synonyms
HSP27 Antibody, Clone 8A7
UNSPSC
12352203
UN Code
Non-hazardous
Hazard Statement
Non-hazardous
Gene ID
3315
Swiss Prot
P04792
Accession Number
NP_001532.1
Cellular Locus
Cytoplasm | Nucleus | Cytoskeleton | Spindle
Host
Mouse
Species Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea Pig, Hamster, Sheep
Immunogen
Human HSP27 peptide
Target
HSP27
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
8A7
Conjugation
Unconjugated
Type
Monoclonal
Applications
WB | IHC | ICC/IF | IP | FCM | FACS
Validated Applications
WB, IHC, ICC/IF, IP, FCM, FACS
Field of Research
Cell Signaling | Protein Trafficking | Chaperone Proteins | Cell Signaling | Cytoskeleton | Microfilaments | Actin | Actin Assembly | Cell Signaling | Cytoskeleton | Microtubules | Cancer | Tumor Biomarkers | Cardiovascular System | Atherosclerosis | Cardiovascular System | Heart | Contractility | Cancer | Heat Shock
Purification
Protein G Purified
Limit Of Detection
A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Concentration
1 mg/ml
Dilution
WB (1:5000), ICC/IF (1:200), IHC (1:100) ; optimal dilutions for assays should be determined by the user.
Weight
0.012
Buffer
PBS, 50% glycerol, 0.09% sodium azide
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Welch W.J. (1985) J Biol. Chem. 260: 3058-3062. 2. Kim K.K., Kim R., and Kim S. (1998) Nature 394(6693): 595-599. 3. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 4. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 5. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70. 6. Welsh M.J., Wu W., Parvinem M., and Gilmont R.R. (1996) Biol. Of Reprod. 55: 141-151. 7. Sarto C. Binnz P.A. and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 8. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6. 9. Jia, Y. et al. (2001) J. Biol. Chem. 276(43):39911-39918.
Shipping Conditions
Blue Ice or 4ºC
Storage Conditions
-20ºC
Specificity
Detects ~25kDa or ~27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.
Background Reference 01
1. Welch W.J. (1985) J Biol. Chem. 260: 3058-3062. 2. Kim K.K., Kim R., and Kim S. (1998) Nature 394 (6693) : 595-599. 3. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 4. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 5. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19) : 1558-70. 6. Welsh M.J., Wu W., Parvinem M., and Gilmont R.R. (1996) Biol. Of Reprod. 55: 141-151. 7. Sarto C. Binnz P.A. and Mocarelli P. (2000) Electrophoresis. 21 (6) : 1218-26. 8. Arrigo A.P. (2005) J Cell Biochem. 94 (2) : 241-6. 9. Jia, Y. et al. (2001) J. Biol. Chem. 276 (43) :39911-39918.
Species
Human | Mouse | Rat | Bovine | Dog | Guinea Pig (Cavia porcellus) | Hamster | Sheep
Location
Cytoplasm | Nucleus | Cytoskeleton | Spindle
Isotype
IgG1 Kappa
Immunogen Species
Human

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