HSP27 Antibody

• Background:

  HSP27, a member of the small heat shock protein (sHSP) family, is an abundant and ubiquitously expressed molecular chaperone found in both normal and cancerous human cells. Structurally, HSP27 features a conserved α-crystallin domain at the C-terminus and a less conserved N-terminal WD/EPF domain, which is essential for forming high-molecular-weight oligomers. These oligomers, composed of 8–40 monomers, are critical for HSP27’s chaperone activity—larger assemblies exhibit strong anti-aggregation properties, while dimers are inactive. Under non-stress conditions, HSP27 resides in the cytoplasm but rapidly translocates to the nucleus in response to cellular stress, where it may help stabilize DNA and nuclear membranes. Functioning as an ATP-independent chaperone, HSP27 prevents protein aggregation and stabilizes partially unfolded proteins, facilitating their refolding via the HSP70 complex. In neuroscience, HSP27 is increasingly recognized for its role in protecting neurons from proteotoxic stress, oxidative damage, and apoptosis—key features of neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS. It inhibits apoptotic signaling by blocking the cytochrome c/Apaf-1/procaspase-9 complex and may also influence cytoskeletal dynamics through interactions with actin and myosin. HSP27 expression is tightly regulated by phosphorylation, which promotes the formation of large, active oligomers. Its involvement in stress response, apoptosis inhibition, and cell differentiation highlights its therapeutic potential in neurodegenerative disease research and intervention.

• Description:

  Rabbit Anti-Human HSP27 Polyclonal

• Specifications:

  Detects ~27kDa.

• Product Name Alternative:

  HSPB1, HSP27, HSP25, HSP28, Heat shock protein beta-1, Heat shock 27 kDa protein, MKBP, DMPK-binding protein, SRP27, 28kDa heat shock protein, CMT2F

• UNSPSC:

  12352203

• UN Code:

  Non-hazardous

• Hazard Statement:

  Non-hazardous

• Gene ID:

  3315

• Swiss Prot:

  P04792

• Accession Number:

  NP_001531.1

• Cellular Locus:

  Cytoplasm | Nucleus

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Shark, Dog

• Immunogen:

  Human HSP27, His tagged

• Target:

  HSP27

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal

• Applications:

  WB | ICC/IF | FCM | FACS | IHC

• Validated Applications:

  WB, ICC/IF, FCM, FACS, IHC

• Field of Research:

  Cell Signaling | Protein Trafficking | Chaperone Proteins | Cell Signaling | Cytoskeleton | Microfilaments | Actin | Actin Assembly | Cell Signaling | Cytoskeleton | Microtubules | Cancer | Tumor Biomarkers | Cardiovascular System | Atherosclerosis | Cardiovascular System | Heart | Contractility |Cancer | Heat Shock

• Purification:

  Protein A Purified

• Detection Range:

  A 1:2000 dilution of SPC-106 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

• Concentration:

  1.38 mg/ml

• Dilution:

  WB (1:2000), ICC/IF (1:250) ; optimal dilutions for assays should be determined by the user.

• Weight:

  0.2

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394 (6693) : 595-599. 2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19) : 1558-70. 5. Sarto C., Binnz P.A., and Mocarelli P. (2000) Electrophoresis. 21 (6) : 1218-26. 6. Arrigo A.P. (2005) J Cell Biochem. 94 (2) : 241-6.

• Shipping Conditions:

  Blue Ice or 4ºC

• Storage Conditions:

  -20ºC

• Specificity:

  Detects ~27kDa.

• Species:

  Human | Mouse | Shark | Gummy Shark (Mustelus antarcticus) | School Shark (Galeorhinus galeus) | Dog

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm | Nucleus

• Immunogen Species:

  Human