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Mouse Transmembrane protein 53 (TMEM53) ELISA Kit

CAT: 0749-KTE70179-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0749-KTE70179-01Size:48 Tests
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Background
TMEM53 has no paralogs but is predicted to have many orthologs across eukaryotes. The secondary structure of TMEM53 is predicted to consist of alternating pairs of alpha helices and beta sheets.The function of TMEM53 is not fully understood. It contains a domain of unknown function, DUF829, which is approximately 240 amino acids long. This domain has not been found in proteins other than TMEM53 and its orthologs. Based on human and mouse EST profiles and a human tissue GEO profile, TMEM53 appears to be expressed ubiquitously at low levels in both normal and cancerous tissues.Transmembrane protein 53 has no paralogs. It does, however, have orthologs extending throughout eukaryotes, from primates to amoeba.
Description
This Mouse Transmembrane protein 53 (TMEM53) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM53.
CAS Number
7732-18-5
Product Name Alternative
TMEM53; RP4-678E16.2; FLJ22353; NET4; novel DUF829 domain-containing protein
Gene ID
68777
Accession Number
Q9D0Z3
Reactivity
Mouse
Tag
TMEM53
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Mouse Transmembrane protein 53 (TMEM53) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TMEM53. No significant cross-reactivity or interference between Mouse TMEM53 and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Mouse Transmembrane protein 53 (TMEM53) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM53 in samples. An antibody specific for TMEM53 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM53 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM53 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM53 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
TMEM53; RP4-678E16.2; FLJ22353; NET4; novel DUF829 domain-containing protein
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Accession Number URL
http://www.uniprot.org/uniprot/Q9D0Z3
Calibration Range
Please inquire

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