Products for Research Use Only
RegisterLogin

Mouse Thioredoxin (TRX) ELISA Kit

CAT: 0749-KTE70044-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0749-KTE70044-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals.Thioredoxins are characterized at the level of their amino acid sequence by the presence of two vicinal cysteines in a CXXC motif. These two cysteines are the key to the ability of thioredoxin to reduce other proteins. Thioredoxin proteins also have a characteristic tertiary structure termed the thioredoxin fold.The thioredoxins are kept in the reduced state by the flavoenzyme thioredoxin reductase, in a NADPH-dependent reaction. Thioredoxins act as electron donors to peroxidases and ribonucleotide reductase.
Description
This Mouse Thioredoxin (TRX) ELISA Kit employs a two-site sandwich ELISA to quantitate TXN.
CAS Number
7732-18-5
Product Name Alternative
TXN; RP11-427L11.1; DKFZp686B1993; MGC61975; TRX; TRX1
Gene ID
22166
Accession Number
P10639
Reactivity
Mouse
Tag
TXN
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Mouse Thioredoxin (TRX) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TXN. No significant cross-reactivity or interference between Mouse TXN and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Mouse Thioredoxin (TRX) ELISA Kit employs a two-site sandwich ELISA to quantitate TXN in samples. An antibody specific for TXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
TXN; RP11-427L11.1; DKFZp686B1993; MGC61975; TRX; TRX1
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Accession Number URL
http://www.uniprot.org/uniprot/P10639
Calibration Range
Please inquire

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
KTE60113-01Human Thioredoxin (TRX) ELISA Kit
KTE70042-01Mouse Thioredoxin reductas (TRXR) ELISA Kit
KTE62634-01ELISA kit for Human Thioredoxin reductase (TRXR)
KTE100995-01ELISA kit for Rat Thioredoxin reductase (TRXR)
KTE70043-01Mouse Thioredoxin-interacting protein (TXNIP) ELISA Kit
KTE70142-01Mouse Thioredoxin-related transmembrane protein 1 (TMX1) ELISA Kit
KTE70139-01Mouse Thioredoxin-related transmembrane protein 4 (TMX4) ELISA Kit
KTE70141-01Mouse Thioredoxin-related transmembrane protein 2 (TMX2) ELISA Kit
KTE70694-01ELISA kit for Mouse Thioredoxin-dependent peroxide reductase, mitochondrial (PRDX3)
KTE60112-01Human Thioredoxin-interacting protein (TXNIP) ELISA Kit

Alternative Products

CatalogName
HY-P73432-01Thioredoxin/TRX, Mouse
GTR18870045-01Mouse Thioredoxin (Trx) ELISA Kit
JOT-EK0131Mo-01Mouse Thioredoxin,Trx ELISA KIT
JL13059-01Mouse Thioredoxin (Trx) ELISA Kit
H9181Trx (Thioredoxin) Mouse ELISA Kit
KLM0131GENLISA™ Mouse Thioredoxin (Trx) ELISA
RK03259Mouse Thioredoxin ELISA Kit (Trx)
EIA06479mMouse Thioredoxin (Trx) ELISA Kit
EKE61795-01Mouse Trx (Thioredoxin) ELISA Kit
CSB-E09730m-01Mouse Thioredoxin, Trx ELISA Kit

Popular Products