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Human Estrone (E1) ELISA Kit

CAT: 0749-KTE63120-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0749-KTE63120-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Employs a two-site sandwich ELISA to quantitate Estrone in samples.
CAS Number
7732-18-5
Reactivity
Human
Tag
Estrone
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
1.0 pmol/L
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
Human Estrone (E1) ELISA Kit employs a two-site sandwich ELISA to quantitate Estrone in samples. An antibody specific for Estrone has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Estrone present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human Estrone detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of Estrone bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Calibration Range
30 pmol/L-480 pmol/L

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