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Human 27-hydroxycholesterol ELISA Kit

CAT: 0749-KTE62711-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0749-KTE62711-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
27-Hydroxycholesterol (27-HC) is an endogenous oxysterol with multiple biological functions, including activity as a selective estrogen receptor modulator (SERM) (a mixed, tissue-specific agonist-antagonist of the estrogen receptor (ER) ) and as an agonist of the liver X receptor (LXR) . It is a metabolite of cholesterol that is produced by the enzyme CYP27A1. A link between high cholesterol and breast cancer has been identified, and it has been proposed that this is due to 27-HC production by CYP27A1. Because of its estrogenic action, 27-HC stimulates the growth of ER-positive breast cancer cells, and has been implicated in limiting the effectiveness of aromatase inhibitors in the treatment of breast cancer. As such, identified CYP27A1 inhibitors, including the marketed drugs anastrozole, fadrozole, bicalutamide, dexmedetomidine, ravuconazole, and posaconazole, have been proposed as potential adjuvant therapies in ER-positive breast cancer.
Description
This Human 27-hydroxycholesterol ELISA Kit employs a two-site sandwich ELISA to quantitate 27HC.
CAS Number
7732-18-5
Product Name Alternative
27HC
Reactivity
Human
Tag
27HC
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human 27-hydroxycholesterol ELISA Kit has high sensitivity and excellent specificity for detection of Human 27HC. No significant cross-reactivity or interference between Human 27HC and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Human 27-hydroxycholesterol ELISA Kit employs a two-site sandwich ELISA to quantitate 27HC in samples. An antibody specific for 27HC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any27HC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 27HC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 27HC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
27HC
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Calibration Range
Please inquire

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