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Human Synapsin-1 (SYN1) ELISA Kit

CAT: 0749-KTE60411-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0749-KTE60411-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The synapsin I protein is a member of the synapsin family that are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases.The phosphoprotein plays a role in regulation of axonogenesis and synaptogenesis. The protein serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal.Synapsin I is found in two isoforms of the protein, Synapsin Ia and Synapsin Ib, with Synapsin Ib being a slightly shorter version of the protein.
Description
This Human Synapsin-1 (SYN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYN1.
CAS Number
7732-18-5
Product Name Alternative
SYN1; RP1-230G1.2; SYN1a; SYN1b; SYNI; brain protein 4.1
Gene ID
6853
Accession Number
P17600
Reactivity
Human
Tag
SYN1
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Synapsin-1 (SYN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYN1. No significant cross-reactivity or interference between Human SYN1 and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Human Synapsin-1 (SYN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYN1 in samples. An antibody specific for SYN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
SYN1; RP1-230G1.2; SYN1a; SYN1b; SYNI; brain protein 4.1
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Accession Number URL
http://www.uniprot.org/uniprot/P17600
Calibration Range
Please inquire

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