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ODPB Polyclonal Antibody

CAT: 0749-ABP59639-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP59639-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. PDHB (Pyruvate Dehydrogenase E1 Beta Subunit) encodes the E1 beta subunit. Mutations in PDHB are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for PDHB.
Description
Rabbit Anti-ODPB Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-
Gene ID
5162
Accession Number
P11177
Reactivity
Human, Rat, Mouse
Immunogen
Synthesized peptide derived from part region of human ODPB protein at amino acid sequence of 160-240
Clonality
Polyclonal
Tag
ODPB
Applications
WB, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
39kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
The antibody detects endogenous levels of ODPB protein
Observed Molecular Weight
39
Formulation
Liquid solution
Buffer pH
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000
Other Product Names
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHE1-
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P11177
Isotype
Rabbit IgG

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