HUS1 Polyclonal Antibody

• Background :

  The protein encoded by HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.

• Description :

  Rabbit Anti-HUS1 Polyclonal Antibody

• CAS Number :

  9007-83-4

• Product Name Alternative :

  Checkpoint protein HUS1; hHUS1

• Gene ID :

  3364

• Accession Number :

  O60921

• Reactivity :

  Human, Mouse

• Immunogen :

  Synthesized peptide derived from part region of human HUS1 protein at amino acid sequence of 160-240

• Clonality :

  Polyclonal

• Tag :

  HUS1

• Applications :

  WB, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  30kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  The antibody detects endogenous levels of HUS1 protein

• Observed Molecular Weight :

  30

• Formulation :

  Liquid solution

• Buffer pH :

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000

• Other Product Names :

  Checkpoint protein HUS1; hHUS1

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/O60921

• Isotype :

  Rabbit IgG