CDC42 Polyclonal Antibody

• Background:

  The protein encoded by CDC42 (Cell Division Cycle 42) is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of CDC42 results in multiple transcript variants. Pseudogenes of CDC42 have been identified on chromosomes 3, 4, 5, 7, 8 and 20.

• Description:

  Rabbit Anti-CDC42 Polyclonal Antibody

• Product Name Alternative:

  Cell division control protein 42 homolog; G25K GTP-binding protein

• Gene ID:

  998

• Accession Number:

  P60953

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from part region of human CDC42 protein at amino acid sequence of 80-160

• Clonality:

  Polyclonal

• Tag:

  CDC42

• Applications:

  WB, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  21kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  The antibody detects endogenous levels of CDC42 protein

• Observed Molecular Weight:

  21

• Formulation:

  Liquid solution

• Buffer pH:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000

• Other Product Names:

  Cell division control protein 42 homolog; G25K GTP-binding protein

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/P60953

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG