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Cyclin E1 (phospho Thr77) Polyclonal Antibody

CAT: 0749-ABP56920-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP56920-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The protein encoded by CCNE1 (cyclin E1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of CCNE1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Description
Rabbit Anti-Cyclin E1 (phospho Thr77) Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
CCNE1; CCNE; G1/S-specific cyclin-E1
Gene ID
898
Accession Number
P24864
Reactivity
Human, Rat, Mouse
Immunogen
Synthesized peptide derived from human Cyclin E1 around the phosphorylation site of T77
Clonality
Polyclonal
Tag
Cyclin-E1
Applications
IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Phospho-Cyclin E1 (T77) Polyclonal Antibody detects endogenous levels of Cyclin E1 protein only when phosphorylated at T77.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
Other Product Names
CCNE1; CCNE; G1/S-specific cyclin-E1
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P24864
Isotype
Rabbit IgG

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