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Phospho-Cyclin E1 (Thr77) Polyclonal Antibody

CAT: 0498-bs-3125RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-3125RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Synonyms
CCNE 1; CCNE; CCNE1; Cyclin E1 antibody Cyclin Es; Cyclin Et; CyclinE; G1/S specic cyclin E; CCNE1_HUMAN.
Gene ID
898
Modification Site
Thr77
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-Cyclin E1 (Thr77)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human Cyclin E around the phosphorylation site of Thr77
Applications
WB, ELISA, FCM, IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
898
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit

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