Bub1 Polyclonal Antibody

• Background:

  BUB1 (BUB1 mitotic checkpoint serine/threonine kinase) encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in BUB1 have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.

• Description:

  Rabbit Anti-Bub1 Polyclonal Antibody

• Product Name Alternative:

  BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

• Gene ID:

  699

• Accession Number:

  O43683

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthesized peptide derived from the Internal region of human Bub1 at AA range: 220-300

• Clonality:

  Polyclonal

• Tag:

  Mitotic checkpoint serine threonine-protein kinase BUB1

• Applications:

  IHC, IF, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  Bub1 Polyclonal Antibody detects endogenous levels of Bub1 protein.

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200

• Other Product Names:

  BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/O43683

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG