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PCB Polyclonal Antibody

CAT: 0749-ABP55489-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP55489-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PC encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Description
Rabbit Anti-PCB Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
PC; Pyruvate carboxylase; mitochondrial; Pyruvic carboxylase; PCB
Gene ID
5091
Accession Number
P11498
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from the Internal region of human PCB at AA range: 330-410
Clonality
Polyclonal
Tag
Pyruvate carboxylase mitochondrial
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
120kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
PCB Polyclonal Antibody detects endogenous levels of PCB protein.
Observed Molecular Weight
120
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications.
Other Product Names
PC; Pyruvate carboxylase; mitochondrial; Pyruvic carboxylase; PCB
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P11498
Isotype
Rabbit IgG

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