Products for Research Use Only

TFIIA-α Polyclonal Antibody

CAT: 0749-ABP54686-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP54686-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]) . One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.
Description
Rabbit Anti-TFIIA-α Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
GTF2A1; TF2A1; Transcription initiation factor IIA subunit 1; General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42
Gene ID
2957
Accession Number
P52655
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from the C-terminal region of human TFIIA-α at AA rangle: 250-330
Clonality
Polyclonal
Tag
Transcription initiation factor IIA subunit 1
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
42kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
TFIIA-α Polyclonal Antibody detects endogenous levels of TFIIA-α protein.
Observed Molecular Weight
42
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Other Product Names
GTF2A1; TF2A1; Transcription initiation factor IIA subunit 1; General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P52655
Isotype
Rabbit IgG