COL4A4 Polyclonal Antibody

• Background:

  COL4A4 encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, COL4A4 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease) . Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR.

• Description:

  Rabbit Anti-COL4A4 Polyclonal Antibody

• Product Name Alternative:

  COL4A4; Collagen alpha-4 (IV) chain

• Gene ID:

  1286

• Accession Number:

  P53420

• Reactivity:

  Human, Mouse

• Immunogen:

  Synthesized peptide derived from the Internal region of human COL4A4 at AA rangle: 510-590

• Clonality:

  Polyclonal

• Tag:

  Collagen alpha-4 (IV) chain

• Applications:

  IHC, IF, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  COL4A4 Polyclonal Antibody detects endogenous levels of COL4A4 protein.

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.

• Other Product Names:

  COL4A4; Collagen alpha-4 (IV) chain

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/P53420

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG