DNA pol θ Polyclonal Antibody

POLQ (Polymerase (DNA) Theta) is a Protein Coding gene. Among its related pathways are Platinum Pathway, Pharmacokinetics/Pharmacodynamics and DNA Double-Strand Break Repair. GO annotations related to this gene include nucleic acid binding and damaged DNA binding. An important paralog of this gene is SNRNP200. NA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed: 25642963, PubMed: 25643323) . MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed: 25642963, PubMed: 25643323) . POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed: 25642963) . POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity) . The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed: 25643323) . The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed: 18503084, PubMed: 21050863, PubMed: 22135286) . Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed: 14576298, PubMed: 18503084, PubMed: 19188258, PubMed: 24648516) . Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity) .

Rabbit Anti-DNA pol θ Polyclonal Antibody

9007-83-4

POLQ; POLH; DNA polymerase theta; DNA polymerase eta

10721

O75417

Human, Mouse

Synthesized peptide derived from the N-terminal region of human DNA pol θ at AA rangle: 150-230

DNA polymerase theta

IHC, IF, ELISA

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

1 mg/ml

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Gel pack with blue ice.

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

DNA pol θ Polyclonal Antibody detects endogenous levels of DNA pol θ protein.

Liquid solution

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200

POLQ; POLH; DNA polymerase theta; DNA polymerase eta

WB, IHC, IF, IP, ELISA

Rabbit

http://www.uniprot.org/uniprot/O75417