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Vinculin Polyclonal Antibody

CAT: 0749-ABP52701-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP52701-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Description
Rabbit Anti-Vinculin Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
VCL; Vinculin; Metavinculin
Gene ID
7414
Accession Number
P18206
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human Vinculin around the non-phosphorylation site of Y821
Clonality
Polyclonal
Tag
Vinculin
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
123kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Vinculin Polyclonal Antibody detects endogenous levels of Vinculin protein.
Observed Molecular Weight
123
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:100-300; Not yet tested in other applications.
Other Product Names
VCL; Vinculin; Metavinculin
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P18206
Isotype
Rabbit IgG

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