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Rpb1 Polyclonal Antibody

CAT: 0749-ABP52394-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP52394-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
POLR2A encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Description
Rabbit Anti-Rpb1 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
Gene ID
5430
Accession Number
P24928
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Synthesized peptide derived from human Rpb1 around the non-phosphorylation site of S1619
Clonality
Polyclonal
Tag
DNA-directed RNA polymerase II subunit RPB1
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
250kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Rpb1 Polyclonal Antibody detects endogenous levels of Rpb1 protein.
Observed Molecular Weight
250
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
Other Product Names
POLR2A; POLR2; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P24928
Isotype
Rabbit IgG

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