Products for Research Use Only

Oct-3/4 Polyclonal Antibody

CAT: 0749-ABP52004-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP52004-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
POU5F1 encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of POU5F1 in adult tissues is associated with tumorigenesis. POU5F1 can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Description
Rabbit Anti-Oct-3/4 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
POU5F1; OCT3; OCT4; OTF3; POU domain; class 5, transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3
Gene ID
5460
Accession Number
Q01860
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from the Internal region of human Oct-3/4 at AA range: 160-240
Clonality
Polyclonal
Tag
POU domain class 5 transcription factor 1
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
50kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Oct-3/4 Polyclonal Antibody detects endogenous levels of Oct-3/4 protein.
Observed Molecular Weight
50
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Other Product Names
POU5F1; OCT3; OCT4; OTF3; POU domain; class 5, transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q01860
Isotype
Rabbit IgG