EIF4E Polyclonal Antibody

• Background :

  Eukaryotic translation initiation factor 4E encoded by EIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. EIF4E acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of EIF4E are found on other chromosomes. Alternative splicing results in multiple transcript variants.

• Description :

  Rabbit Anti-eIF4E Polyclonal Antibody

• Product Name Alternative :

  EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

• Gene ID :

  1977

• Accession Number :

  P06730

• Reactivity :

  Human, Mouse, Rat, Monkey

• Immunogen :

  Synthesized peptide derived from human eIF4E around the non-phosphorylation site of S209

• Clonality :

  Polyclonal

• Tag :

  Eukaryotic translation initiation factor 4E

• Applications :

  WB, IHC, IF, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  25kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  EIF4E Polyclonal Antibody detects endogenous levels of eIF4E protein.

• Observed Molecular Weight :

  25

• Formulation :

  Liquid solution

• Buffer pH :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200

• Other Product Names :

  EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/P06730

• CAS Number :

  9007-83-4

• Isotype :

  Rabbit IgG