EIF3ε Polyclonal Antibody

• Background :

  Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.

• Description :

  Rabbit Anti-eIF3ε Polyclonal Antibody

• Product Name Alternative :

  EIF3F; EIF3S5; Eukaryotic translation initiation factor 3 subunit F; eIF3f; Deubiquitinating enzyme eIF3f; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47

• Gene ID :

  8665

• Accession Number :

  O00303

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthesized peptide derived from the Internal region of human eIF3ε at AA range: 50-130

• Clonality :

  Polyclonal

• Tag :

  Eukaryotic translation initiation factor 3 subunit F

• Applications :

  WB, IHC, IF, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  38kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  EIF3ε Polyclonal Antibody detects endogenous levels of eIF3ε protein.

• Observed Molecular Weight :

  38

• Formulation :

  Liquid solution

• Buffer pH :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200

• Other Product Names :

  EIF3F; EIF3S5; Eukaryotic translation initiation factor 3 subunit F; eIF3f; Deubiquitinating enzyme eIF3f; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/O00303

• CAS Number :

  9007-83-4

• Isotype :

  Rabbit IgG