Adducin β Polyclonal Antibody

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.

Rabbit Anti-Adducin β Polyclonal Antibody

9007-83-4

ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta

119

P35612

Human, Mouse, Rat

Synthesized peptide derived from the Internal region of human Adducin β at AA range: 440-520

Beta-adducin

WB, IF, ELISA

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

1 mg/ml

81kD

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Gel pack with blue ice.

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Adducin β Polyclonal Antibody detects endogenous levels of Adducin β protein.

Liquid solution

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.

ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta

WB, IHC, IF, IP, ELISA

Rabbit

http://www.uniprot.org/uniprot/P35612