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53BP1 Polyclonal Antibody

CAT: 0749-ABP50553-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP50553-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53. The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors. Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation. Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression. 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus. The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus. 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.
Description
Rabbit Anti-53BP1 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
TP53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1
Gene ID
7158
Accession Number
Q12888
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human 53BP1 around the non-phosphorylation site of S6
Clonality
Polyclonal
Tag
Tumor suppressor p53-binding protein 1
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
213kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
53BP1 Polyclonal Antibody detects endogenous levels of 53BP1 protein.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
Other Product Names
TP53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q12888
Isotype
Rabbit IgG

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