14-3-3 ε Polyclonal Antibody

CAT: 0749-ABP50542-01Size: 30 µLDry Ice: NoHazardous: No

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CAT#:0749-ABP50542-01Size:30 µL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Background

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for YWHAE.

Description

Rabbit Anti-14-3-3 ε Polyclonal Antibody

CAS Number

9007-83-4

Product Name Alternative

YWHAE; 14-3-3 protein epsilon; 14-3-3E

Gene ID

7531

Accession Number

P62258

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the C-terminal region of human 14-3-3 ε at AA range: 180-260

Clonality

Polyclonal

Tag

14-3-3 protein epsilon

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

29kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

14-3-3 ε Polyclonal Antibody detects endogenous levels of 14-3-3 ε protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.

Other Product Names

YWHAE; 14-3-3 protein epsilon; 14-3-3E

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P62258

Isotype

Rabbit IgG

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