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Cdc25B (phospho Ser323) Polyclonal Antibody

CAT: 0749-ABP50284-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP50284-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. Cell division cycle 25B is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for CDC25B exist.
Description
Rabbit Anti-Cdc25B (phospho Ser323) Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B
Gene ID
994
Accession Number
P30305
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human Cdc25B around the phosphorylation site of S323
Clonality
Polyclonal
Tag
M-phase inducer phosphatase 2
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
70kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Phospho-Cdc25B (S323) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S323.
Observed Molecular Weight
70
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; IF 1:50-200
Other Product Names
CDC25B; CDC25HU2; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P30305
Isotype
Rabbit IgG

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