TLK1 rabbit pAb

• Background:

  Catalytic activity:ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., enzyme regulation:Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-743, potentially by CHK1., function:Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family., similarity:Contains 1 protein kinase domain., subunit:Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B., tissue specificity:Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.

• Description:

  Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. enzyme regulation: Cell-cycle regulated, maximal activity in S-phase. Inactivated by phosphorylation at Ser-743, potentially by CHK1. function: Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by faciliting the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. similarity: Contains 1 protein kinase domain. subunit: Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B. tissue specificity: Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.

• UniProt:

  Q9UKI8

• Swiss Prot:

  Q9UKI8

• Reactivity:

  Human; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human TLK1. AA range:730-779

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

• Storage Conditions:

  -20°C/1 year

• Fragment:

  IgG

• Other Product Names:

  TLK1; KIAA0137; Serine/threonine-protein kinase tousled-like 1; PKU-beta; Tousled-like kinase 1

• Gene ID (Human):

  9874