ATPBD3 rabbit pAb

• Background:

  Function:Plays a central role in 2-thiolation of mcm (5) S (2) U at tRNA wobble positions of tRNA (Lys), tRNA (Glu) and tRNA (Gln) . Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position., pathway:tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis., similarity:Belongs to the ttcA family. CTU1/NCS6/ATPBD3 subfamily., subunit:Component of a complex at least composed of URM1, CTU2/NCS2 and ATPBD3/NCS6. May for a heterodimer with CTU2/NCS2.

• Description:

  Function: Plays a central role in 2-thiolation of mcm (5)S (2)U at tRNA wobble positions of tRNA (Lys), tRNA (Glu) and tRNA (Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. pathway: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. similarity: Belongs to the ttcA family. CTU1/NCS6/ATPBD3 subfamily. subunit: Component of a complex at least composed of URM1, CTU2/NCS2 and ATPBD3/NCS6. May for a heterodimer with CTU2/NCS2.

• Synonyms:

  CTU1; ATPBD3; NCS6; Cytoplasmic tRNA 2-thiolation protein 1; ATP-binding domain-containing protein 3; Cancer-associated gene protein; Cytoplasmic tRNA adenylyltransferase 1

• Gene ID:

  90353

• UniProt:

  Q7Z7A3

• Cellular Locus:

  Cytoplasm.

• Host:

  Rabbit

• Species Reactivity:

  Human, Rat, Mouse,

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human ATPBD3. AA range:291-340

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, ELISA

• Validated Applications:

  WB, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

• Molecular Weight:

  36kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  36 kD

• Subcellular Location:

  Cytoplasm.

• Other Product Names:

  CTU1; ATPBD3; NCS6; Cytoplasmic tRNA 2-thiolation protein 1; ATP-binding domain-containing protein 3; Cancer-associated gene protein; Cytoplasmic tRNA adenylyltransferase 1

• Gene ID (Human):

  90353

• SwissProt (Human):

  Q7Z7A3