Sp3/4 rabbit pAb

• Background:

  This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]

• Description:

  This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010],

• UniProt:

  Q02447/Q02446

• Swiss Prot:

  Q02447/Q02446

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human SP3/4. AA range:671-720

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  82kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  82kD

• Fragment:

  IgG

• Subcellular Location:

  Nucleus. Nucleus, PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.

• Other Product Names:

  SP3; Transcription factor Sp3; SPR-2; SP4; Transcription factor Sp4; SPR-1

• Gene ID (Human):

  6670/6671