Endophilin I rabbit pAb

Endophilin I rabbit pAb

• Description: Domain: An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface. function: Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. miscellaneous: HeLa cells expressing the N-BAR domain of SH3GL2 show tubulation of the plasma membrane. The N-BAR domain binds liposomes and induces formation of tubules from liposomes. The N-terminal amphipathic helix is required for liposome binding. The second amphipathic helix enhances liposome tubulation. similarity: Belongs to the endophilin family. similarity: Contains 1 BAR domain. similarity: Contains 1 SH3 domain. subcellular location: Concentrated in presynaptic nerve terminals in neurons. subunit: Monomer; in cytoplasm. Homodimer; when associated with membranes (By similarity). Interacts with SYNJ1 and DNM1. Interacts with MAP4K3; the interaction appears to regulate MAP4K3-mediated JNK activation. Interacts with PDCD6IP. tissue specificity: Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
• Synonyms: SH3GL2; CNSA2; SH3D2A; Endophilin-A1; EEN-B1; Endophilin-1; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2
• Gene ID: 6456
• UniProt: Q99962
• Cellular Locus: Cytoplasm. Membrane ; Peripheral membrane protein. Early endosome. Cell junction, synapse, presynapse.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthesized peptide derived from Endophilin I . at AA range: 30-110
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
• Molecular Weight: 39kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.