RPA32 (phospho Thr21) rabbit pAb
CAT:
855-ES7061-01
Size:
50 μL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
RPA32 (phospho Thr21) rabbit pAb
- Description: Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2. subcellular location: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. subunit: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.
- Synonyms: RPA2; REPA2; RPA32; RPA34; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34
- Gene ID: 6118
- UniProt: P15927
- Cellular Locus: Nucleus. Nucleus, PML body. Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner..
- Host: Rabbit
- Species Reactivity: Human, Mouse
- Immunogen: The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Thr21. AA range:10-59
- Clonality: Polyclonal
- Validated Applications: WB, IHC, IF, ELISA
- Stability: 1 year
- Concentration: 1 mg/mL
- Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
- Molecular Weight: 35kD
- Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.