Raf-1 (phospho Ser296) rabbit pAb

CAT: 0855-ES6981-01Size: 50 µLDry Ice: NoHazardous: No

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Background

This gene is the cellular homolog of viral raf gene (v-raf) . The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]

Description

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],

Product Name Alternative

RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

UniProt

P04049

Swiss Prot

P04049

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser296. AA range:271-320

Target

Raf-1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

74kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

74kD

Fragment

IgG

Subcellular Location

Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.

Other Product Names

RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Gene ID (Human)

5894

UniProtKB · P04049

RAF proto-oncogene serine/threonine-protein kinase

RAF1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P04049
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: RAF1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 2.7.11.1
Processing: —
Secondary accessions: B0LPH8, B2R5N3, Q15278, Q9UC20

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMetal-bindingNucleotide-bindingZinc

Disease

CardiomyopathyDeafnessDisease variantProto-oncogene

Cellular component

Cell membraneCytoplasmMembraneMitochondrionNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

MethylationPhosphoprotein

Domain

Zinc-finger

Related Products

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ES5954-01	A-Raf (phospho Ser299) rabbit pAb
ES4596-01	Chk1 (phospho Ser296) rabbit pAb
ES6979-01	Raf-1 (phospho Ser642) rabbit pAb
ES6983-01	Raf-1 (phospho Thr269) rabbit pAb
ES6978-01	Raf-1 (phospho Ser301) rabbit pAb
ES1443-01	Raf-1 (phospho Ser338) rabbit pAb
ES6984-01	Raf-1 (phospho Ser289) rabbit pAb
ES6980-01	Raf-1 (phospho Tyr341) rabbit pAb
ES1399-01	Raf-1 (phospho Ser259) rabbit pAb
ES6982-01	Raf-1 (phospho Ser621) rabbit pAb

Alternative Products

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GTR18568675	Raf-1 (phospho Ser296) Antibody
GTR18052551-01	Raf-1 (phospho Ser296) rabbit pAb Antibody
E28PP0690	Raf-1 (phospho Ser296) Rabbit Polyclonal Antibody
JOT-AP07731-01	Raf-1(Phospho Ser296) Polyclonal Antibody
MBS8520464-01	Raf-1 (phospho Ser296) Polyclonal Antibody
MBS8511247-01	Phospho-C-RAF (Ser296) Antibody
MBS8585219-01	C-RAF (Phospho-Ser296) Antibody
AF3063-BP	Phospho-C-RAF (Ser296) Peptide
OAAF07467-100UG	C-RAF Antibody (Phospho-Ser296)
GTR18079958-01	Raf (phospho Ser296) Polyclonal Antibody