ATR (phospho Ser428) rabbit pAb

ATR (phospho Ser428) rabbit pAb

• Description: The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq, Jul 2008],
• Synonyms: ATR; FRP1; Serine/threonine-protein kinase ATR; Ataxia telangiectasia and Rad3-related protein; FRAP-related protein 1
• Gene ID: 545
• UniProt: Q13535
• Cellular Locus: Nucleus. Chromosome. Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: The antiserum was produced against synthesized peptide derived from human ATR around the phosphorylation site of Ser428. AA range:394-443
• Clonality: Polyclonal
• Validated Applications: IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.